Department
Natural Sciences
Document Type
Poster
Abstract
The gene CDT1 is believed to be important for cadmium tolerance in plants. Here, we describe the production of a reporter construct to study CDT1 gene expression in the model plant Arabidopsis thaliana. We used Gibson assembly to insert the CDT1 promoter sequence into a plasmid containing the GUS reporter gene. The Gibson Assembly procedure provides an efficient method to join multiple DNA fragments. The resulting plasmid was transformed into E. coli using a heat-shock protocol. We confirmed successful transformations using colony PCR. Further DNA sequence analysis indicated that the CDT1prom-GUS plasmid has successfully been built! Next the CDT1prom-GUS construct will be introduced into Agrobacterium for plant transformation. Ultimately, we will create transgenic plants in which we can visualize CDT1 expression using the GUS reporter.
Publication Date
Spring 4-9-2026
Recommended Citation
Remember to check citations for accuracy before including them in your work.
Dem, Ebrima, "Genetic modification of Arabidopsis thaliana to investigate cadmium tolerance mechanisms" (2026). Student Scholarship. 50.
https://metroworks.metrostate.edu/student-scholarship/50
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
Comments
Spring 2026: Student Research Conference
Best Overall, 1st Place – Ebrima Den